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A protein produced by the B-cells of the immune system which binds to a particular antigen, a foreign substance to the body.
176 questions
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What affinity does an antibody need to show up on a western blot?
I am trying to find some reference for what affinity (however defined, but ideally $K_D$) an antibody needs to be useful as a primary antibody on a western blot.
Even better would be any sort of guide ...
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Is it possible to obtain the sequences from polyclonal antibody protein mixture via mass spec?
We have been using pAbs from an immunized goat for quite a few years but now the goat died. We only have the antigen affinity purified pAb proteins. Is it possible to obtain the sequences?
We have ...
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3
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2
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Do we already have the necessary code for antibodies against various diseases?
Whenever we get exposed to a particular antigen, we form antibodies against it. Doesn't this suggest that we already have the genetic code to code an antibody against that particular antigen? If not ...
3
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answer
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Neutralization Assay: Comparision of IC50 values between different viral loads
I've been performing Neutralization assays for HIV pseudovirus against a dilution series of different antibodies, and was wondering whether the viral load influences the measured IC50.
My specific ...
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2
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1
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Finding antibodies sequence online
Not sure if this is the right section to ask this question but here we go.
I want to find human GPCR antibody sequences online and download them. I had some trouble finding good platforms with good ...
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What could be the possible reason(s) for background staining in DAB based Immunohistochemistry of paraffin embedded tissues?
I have been having trouble with immunohistochemistry of mouse pancreas tissue for some time now. I observe that while there is good and specific staining of the antibody, there is a lighter shade of ...
user81098
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132
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Do antibodies binding to the same epitope have similar electrophoretic mobility?
Serum protein electrophoresis is a commonly used blood test in medicine. It is often used for detection of paraproteins in the gamma-globulin region. If there is a narrow band with sharp borders ...
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2
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1
answer
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Improving contrast between dot and paper in dot blot
Currently using dot blot to attempt to determine if a serum contains antibodies for some reagents I am testing.
I pipetted samples as 10-5ul dots on whatman paper.
Incubation steps were for 1 hour ...
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0
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1
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Why do monoclonal and polyclonal antibodies behave differently as labeled secondary antibodies?
According to the MBL guide to generating antibodies, the differences between monoclonal and polyclonal antibody extend beyond the fact that the latter is heterogeneous, binding to various epitopes for ...
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1
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1
answer
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Why are camelid-derived nanobodies called VHH (variable heavy domain of heavy chain)?
Single-domain antibodies (or nanobodies) derived from camelid heavy-chain antibodies are called VHH antibodies, where VHH stands for "variable heavy domain of heavy chain". I assume the ...
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1
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Monoclonal Antibodies
In this paper, the authors say:
We demonstrate that for a considerable number of eplets, the
antibody-verified status is solely based on polyclonal serum
reactivity of multiparous women or on ...
2
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I need help identifying the source of my contamination in my viral titration experiments
I am relatively new to what I'm currently working with and as the title suggests, I am currently having some issues with my experiment (viral titration).
So, what I have been doing is the following:
...
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1
vote
1
answer
118
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Do all monoclonal antibodies have a blocking effect?
I find a mAb targeting protein A, which has beed validated to work in wb/IHC/IP or antibody conjugating drugs. I'm wondering if this mAb could be used for blocking the protein A from binding with ...
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1
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Why don't each of T and B cells make two different TCRs/antibodies, except for the class switch?
I have not been able to find any literature that clearly states this, but if I understand correctly, T and B cells are diploid.
If so, there are two sets of genomes, and if both are TCR/VDJ ...
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2
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1
answer
408
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What is the difference between monoclonal and polyclonal antibodies?
I'm having trouble to see a difference between monoclonal and polyclonal antibodies. The way I understand it:
Monoclonal antibodies are produced by the same "clone" or "line" of B-...
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